Research Assistant – San Francisco, CA

Our lab aims to understand the mechanisms of cardiac arrhythmias and how those relate to gross cardiac fibrosis and disease on an electrophysiological and molecular level. The Research Assistant will be involved in all aspects of the research from disease creation to whole tissue mapping to cell line generation. With supervision, the individual will also be responsible for analyzing lines of mice with mutations in central genes involved in cardiac development and diseases. This work involves a variety of developmental biology techniques including mouse/rat husbandry and surgery, large animal surgery/husbandry, collecting and processing histologic specimens, performing in situ hybridization and immunohistochemistry, and processing and analyzing images.

Under supervision, the Research Assistant will also perform a variety of molecular biology, cell biological and biochemical techniques to analyze samples. These techniques include restriction enzyme digestion and mapping, gel electrophoresis of nucleic acid, preparation of DNA, RNA and protein, Southern, Northern and Western blotting, PCR, cell culture and FACS sorting. In conjunction with the basic research in the primary lab we also work with a clinical biospecimen banking study. In this aspect of the job the Research Assistant will work with the research manager and Principal Investigator (PI) to assist with receiving and processing human biological samples such as peripheral blood and saliva. The individual will help with the DNA/RNA extractions and sample aliquoting/plating. This will require precise, careful, technique as we will be barcoding and storing large amounts of samples for long-term future use. This position will also include some administrative and organizational duties; freezer monitoring and other miscellaneous duties as assigned.

The job will involve the ability to use well developed fine motor skills and/or surgical skills.

DIVISIONS OF CARDIOLOGY
The Division of Cardiology is one of the largest clinical, research and training divisions of the Department of Medicine (DOM) at UCSF. Within the Division are sub-specialty sections for: Adult Congenital Heart Disease; Advanced Heart Failure, Transplant, and Pulmonary Hypertension; Cardiac Electrophysiology; Echocardiography and Cardiac Imaging; General Cardiology, Interventional Cardiology; and Prevention. The Division runs several clinical practices in multiple sites, conducts basic and clinical research, and educates medical students, residents, clinical fellows and postdoctoral scholars through Accreditation Council for Graduate Medical Education (ACGME) as well as non-ACGME training programs. In addition, the Division has significant and complex financial and administrative relationships with DOM and the UCSF Medical Center, as well as large patient care programs in the sections noted above, large clinical, Federal, and privately supported research programs and six faculty laboratories.

The Division has 45 full-time faculty, four non-faculty academics, 36 clinical fellows, thee post-doctoral research fellows, seven WOS faculty, two MSP physicians and 57 research support and administrative staff. The Division currently expends over $22 million per year in pursuit of its clinical, research, training, and service missions. While the Division is primarily split between the Parnassus and Mission Bay campuses, fellows have rotations at the Zuckerberg San Francisco General Hospital (ZSFG) and the San Francisco Veterans Affairs Medical Center (SFVAMC).

ABOUT UCSF

The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10-campus UC system dedicated exclusively to the health sciences.

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